Natural Factors	The entire structure of nature is arrayed against the creation of life. Complex chemical compounds in cells are in reduced state, Oxygen (O2) degrades them. Laws of Thermodynamics are opposed to life. Dextrorotatory forms destroy function of proteins. Statistically impossible for life to generate spontaneously.
Simple Life	A one celled bacterium (e.g. E. coli) is estimated to have over 2,000 highly specific and necessary enzymes. The equivalent information of 100 million pages of the Encyclopedia Britannica (1012 bits of data). The worlds largest libraries (British Museum, Oxford Bodleian Library, New York Public Library, Harvard Widener Library and  Moscow Lenin Library) have about 10 million volumes, or 1013 bits of data.
The Chicken or the Egg Problem	To formulate an adequate hypothesis for the origin of genetic code, we must deal with the fact that genetic code has no function unless it is translated (i.e. unless it leads to synthesis of the proteins laid down by the code). But, Monod points out, the machinery by which the cell translates the code "consists of at least 50 different macromolecular components which are themselves coded in DNA." Thus the code cannot be translated except by using certain products of its translation.
Origin of Genetic Code	Dickerson: "The evolution of the genetic machinery is the step for which there are no laboratory models, hence we can speculate endlessly, unfettered by inconvenient facts."
All-or-None Principle	For life to exist, there is some minimal set of ordered processes that must all work at the same time or in sequence in time. If one fails because of an error in its formation, the whole cell dies or fails to live. The immensity of this minimal set is rarely appreciated. Van Rensselear Potter states "It is possible to hazard a guess that the number is not less than 1,000, but whether it is 3,000 or 10,000 or greater is anyone guess."
Natural Selection	It cannot be invoked prior to life existing. Dobzhansky says "Natural selection is differential reproduction, organism perpetuation. In order to have natural selection, you have to have self-reproduction or self-replication and at least two distinct self-replicating units or entities... Prebiologic natural selection is a contradiction of terms."
Primitive Atmosphere	The origin of life theories require that the earth's atmosphere be radically different than now. It must have CH4, CO, CO2 NH3, H2 and H2O instead of O2, N2 and H2O. But there is no evidence for such an atmosphere. Davidson says that no evidence at all; Nelson says no geochemical evidence of CH4; Keosian says "There is no general agreement on what represents primitive earth conditions..."